Groovy Map containing information of the probe targets generated from the panel sequences with opt track
e.g. [ id:'breast_cancer_probe_panel_sequences' ]
probe_targets:file
Generated probe targets
*.tsv
meta2:map
Groovy Map containing information of the forward oriented fasta generated with the probes panel sequences ‘opt flip’
e.g. [ id:'breast_cancer_probe_panel_sequences' ]
fwd_oriented_probes:file
The forward oriented fasta file
*.fa
gene_synonyms:file
Gene synonyms that may have been counted as off-targets but simply differ in name (optional input)
*.csv
Output
name:type
description
pattern
summary
meta:map
Groovy Map containing summary of the forward oriented probes generated with the panel sequences ‘opt flip and track’
e.g. [ id:'breast_cancer_probe_panel_sequences' ]
${meta.id}/collapsed_summary.tsv:file
tsv file containing the summary stats
*.tsv
versions
versions.yml:file
File containing software versions
versions.yml
Tools
opt
GPL-3.0 license
opt is a simple program that aligns probe sequences to transcript sequences to detect potential off-target probe activity
